What if my yield of CUT&Tag DNA is too low to see on an Agilent Bioanalyzer or TapeStation system?

Due to the expected and typically low yield of CUT&Tag DNA, we recommend using all 30 µL of the CUT&Tag DNA sample (from Section VI of the CUT&Tag protocol #77552) for library amplification. While CUT&Tag DNA libraries generated for histone modifications typically show some signal on the Agilent Bioanalyzer or TapeStation systems, libraries generated for non-histone proteins such as transcription factors and cofactors often have very weak or even no visible signal on Bioanalyzer or TapeStation systems, but they still generate NGS results with high-mapping rates, high numbers of identified binding peaks, and decent signal-to-noise ratios across the whole genome. 对于 Bioanalyzer 或 TapeStation 系统不能识别文库平均大小的 CUT&Tag 文库，我们建议使用 900 bp 的大小有意汇集比正常产率文库更多的低产率文库。

Last updated: 2024 年 9 月 12 日