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Results (653)

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:

  • Cleaved Caspase-3 (Asp175) Antibody #9661
  • Cleaved ...

    How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

    Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchanged. However, the abs...

    CUT&RUN 是否偏向于常染色质或异染色质?

    We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly ...

    Are the bands detected by your MT1-MMP (D1E4) Rabbit mAb #13130 the precursor (62 kDa) and active (50 kDa) forms?

    Our MT1-MMP (D1E4) Rabbit mAb #13130 was produced with an antigenic peptide corresponding to residues surrounding Met293 of human MT1-MMP protein (see UniProt ID #P50281; https://www.uniprot.org/unipr...

    Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?

    No, the use of Fc block is not required when using F(ab') or F(ab')2 antibody fragments in live cell flow cytometry assays, as removal of the Fc domain eliminates the interaction between...

    Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?

    During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...

    Can the Senescence β-Galactosidase Staining Kit #9860 be used with frozen tissue?

    While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell ...

    我可以使用哪些对照物来显示 CUT&RUN 实验正在运行？

    Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our 

    My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

    Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

    Why can't spike-in DNA be used in CUT&Tag assays?

    Tagmentation takes place within the nuclei. To minimize non-specific background, any excess Tn5 enzyme is washed away prior to activation. Similarly, spike-in DNA introduced into the reaction woul...

    Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

    Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...

    What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?

    We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&Tag assay is worki...

    What is an appropriate positive control to include in this SignalStar™ assay? 是否需要多个对照？

    Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som...

    Is it possible to perform CUT&Tag on samples like heart, brain, or developing mouse tissues?

    可以。We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each CUT&Tag reaction...

    How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?

    对于第 1 轮成像，在完成染色后 8 小时内成像时，染色应显示稳定信号。For Imaging Round 2, imaging should be performed as close to the completion of staini...

    Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

    We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. We would recommend β-Actin (13E5)...

    I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. 我还能以某种方式在我的检测板中使用它吗？

    SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...

    Does your Insulin (C27C9) Rabbit mAb #3014 detect proinsulin?

    Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; https://www.uniprot.o...

    Is PTMScan® compatible with SILAC?

    PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ...

    您建议哪种 DNA 文库制备试剂盒用于 CUT&Tag 测定法？

    We recommend using our CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415 to make DNA libraries for C...

    Can my slides be used for other assays after SignalStar™ Multiplex IHC?

    SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ...

    Can I freeze the PTMScan® HS Magnetic Immunoaffinity Beads?

    The PTMScan HS Magnetic Immunoaffinity Beads should not be frozen. We recommend storing this product at 4C. However, we have performed functional testing by IAP and subsequent mass spec analysis after...

    Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?

    When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spe...

    How many LC-MS/MS injections are obtained from a PTMScan® enrichment?

    Each PTMScan® enrichment contains sufficient peptides for three to four LC-MS/MS runs. Therefore, one enrichment can produce enough peptides for technical replicates and enough remaining peptides for ...

    Can I autoclave the 6-Tube Magnetic Separation Rack #7017?

    No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the rack with 10% bleach...

    What is the difference between the discontinued anti-rabbit and anti-mouse PE secondary antibodies #8885 & #8887 and their replacements, #79408 & #59997

    We have decided to no longer support the anti-rabbit and anti-mouse PE secondary antibodies, #8885 and #8887. We have developed two new secondaries which prove to be superior to the discontinued produ...

    What is the extinction coefficient of my rabbit antibody?

    The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.