Do you test your antibodies for co-immunoprecipitation (co-IP)?

While we do not specifically test our antibodies using co-IP, we expect IP-approved antibodies to work well for co-IP experiments. As each protein-protein interaction is unique, optimization of lysis conditions and the inclusion of proper controls are necessary for a successful experiment. More information on experimental design for IP and co-IP experiments can be found here. 

For co-IPs, we suggest blotting for the target protein first to confirm pull-down. Once confirmed, blotting for additional proteins in the complex can be performed. If your protein of interest runs at a size similar to the heavy or light chain of IgG, we recommend the use of a conformation-specific or heavy/light chain-specific secondary antibody to avoid masking of the target signal. Additional recommendations for IP and co-IP controls can be found here for your reference. 
 

Last updated: 2024 年 9 月 12 日