CASC5 Antibodies

Target Information

Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1. Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore. In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I. In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments. Component of the KNL1 complex composed of KNL1 and ZWINT (Probable). Part of the ten-subunit outer kinetochore KMN network that includes the KNL1, MIS12 and NDC80 complexes; a bioriented kinetochore contains approximately 150 copies of the network. Interacts (via C-terminus) with the MIS12 complex subunits NSL1 (via C-terminus), PMF1 and DSN1; the interaction is direct (Probable). Interacts (via N-terminal region) with BUB1B (via BUB1 N-terminal domain); the interaction is direct and is required for cell cycle arrest upon activation of the mitotic spindle assembly checkpoint. Interacts (via N-terminal region) with BUB1 (via BUB1 N-terminal domain); the interaction is direct. Interacts with the protein phosphatase PP1 subunit PPP1CA; the interaction is direct and mutually exclusive with binding to microtubules. Interacts with the protein phosphatase PP1 subunit PPP1CC; the interaction is direct and mutually exclusive with binding to microtubules. 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.

Alternate Names

AF15q14; ALL1-fused gene from chromosome 15q14; ALL1-fused gene from chromosome 15q14 protein; Blinkin; blinkin, bub-linking kinetochore protein; Bub-linking kinetochore protein; cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CASC5; CT29; D40; hKNL-1; hSpc105; KIAA1570; kinetochore null 1 homolog; Kinetochore scaffold 1; Kinetochore-null protein 1; KNL1; MCPH4; microcephaly, primary autosomal recessive 4; PPP1R55; Protein CASC5; Protein D40/AF15q14; protein phosphatase 1, regulatory subunit 55; Spc7