Why is the expression of my target protein very low in my InTraSeq™ experiment?

有时，单细胞数据集中出现双联体和多重联体会影响 FeaturePlot 中蛋白质可视化结果的准确性。Using a maximum cutoff (max.cutoff) bypasses the observed high level of protein expression from outliers and allows clear visualization. Use a large value for this parameter, such as "q90" or higher ("q95", "q99", etc.) as appropriate for your experiment. 参见以下粗体示例：
 

FeaturePlot(object, features = c("protein_Phospho-S6(Ser235-236)-Ab"), max.cutoff = “q90”, min.cutoff = "q5")

阅读已发表的文献和挖掘公共数据库也很重要，以确保您的蛋白质在您的样本中表达，并了解哪些条件可能改变这种表达。

如果使用未经 InTraSeq 验证的抗体，抗体结合不足可能会影响可视化结果的准确性。Use CST® InTraSeq-validated antibodies in your single-cell experiments and follow the InTraSeq protocol precisely to ensure reliable and reproducible results.

Last updated: 2024 年 9 月 18 日