Why does your ROBO2 (E4M6D) Rabbit mAb #45568 detect 2 bands by western blot?

We see two bands migrating at 180 and 220 kDa in both endogenous and over-expressed lysates. We suspect these are differentially glycosylated forms of ROBO2 (UniProt ID Q9HCK4; https://www.uniprot.org/uniprot/Q9HCK4#ptm_processing).

Last updated: March 31, 2026