Why does the Calpain 1 Large Subunit (Mu-type) Antibody #2556 detect two bands by western blot?

Our Calpain 1 Large Subunit (Mu-type) Antibody #2558 detects endogenous levels of total calpain 1 (large subunit) protein. The antibody detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28. The intensity of the cleaved band can vary depending on the species and type of cell lysate used.

Last updated: October 6, 2025