Why am I seeing dark background all over my western blot when using your Phospho-p53 (Ser15) Antibody #9284?

It is critical that our Phospho-p53 (Ser15) Antibody #9284 be diluted into 5% w/v non-fat dry milk, 1X TBS, 0.1% Tween^® 20 and incubated at 4°C with gentle shaking, overnight. Diluting the antibody in BSA will result in high background on the blot.

Last updated: October 6, 2025