Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?

When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spectra of DRAQ5 and DyLight 680 overlap, and the signal produced by each dye cannot be distinguished.

Last updated: October 6, 2025