What procedure do you recommend for antigen retrieval with EDTA?

We recommend using a microwave to bring slides to a boil in 1 mM EDTA, pH 8.0 followed by 15 minutes at sub-boiling temperature. Typically the power level must be adjusted during the 15 minute period so as to prevent excessive buffer loss. Slides do not need to be cooled on the bench top, but can be rinsed in running dH2O.

Last updated: October 6, 2025