What is the difference between ChIP and CUT&RUN?

There are several differences between ChIP and CUT&RUN. CUT&RUN is free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a much faster and more efficient method for enriching protein-DNA interactions and identifying target genes. 和 ChIP 中碎裂所有细胞染色质不同，CUT&RUN 利用一种染色质抗体靶向消化方法，从而使背景信号比 ChIP 实验低得多。As a result, CUT&RUN requires only 1/10th the sequencing depth that is required for ChIP-seq assays.

Last updated: 2024 年 9 月 12 日