What is the recommended gradient length for PTMScan® enrichments?

For PTMScan® enrichments, we recommend a liquid chromatography (LC) gradient length of 90 minutes and replicate injections for each enrichment. Therefore, four PTMScan enrichments will have a total of eight liquid chromatography-mass spectrometry (LC–MS)/MS runs. For the immobilized metal affinity chromatography (IMAC) based phosphopeptide enrichments, the sample complexity requires a longer gradient length of 120 minutes, also performed with replicate injections for each sample.
 

Last updated: March 25, 2026