What are the band sizes detected with the FLIP (D5J1E) Rabbit mAb #56343 in human and mouse extracts by western blot?

Human samples will have 55 kDa and 25 kDa bands, representing the long and short isoforms (FLIP-L, FLIP-S). A third isoform, FLIP-R, was originally identified in Raji cells and migrates at approximately 25 kDa [see Djerbi, M. et al. (2001) Scand. J Immunol. 54, 180-9 (PMID: 11439165; https://www.ncbi.nlm.nih.gov/pubmed/1439165) and Golks A. et al. (2005) J. Biol. Chem. 280, 14507-13 (PMID: 15701649; https://www.ncbi.nlm.nih.gov/pubmed/15701649)]. Mouse FLIP is alternatively spliced, where only the long isoform is generated and, in some rare cases, the FLIP-R isoform may be observed. Frequently, the shorter isoforms undergo proteasomal degradation in mice and are not detected by western blot.

 

Last updated: 2024 年 9 月 12 日