我是否应该担心自己的伴刀豆球蛋白 A 珠在自身一些 CUT&Tag 实验中“板结在一起”？

Concanavalin A beads can clump together rather easily, especially when cells become lysed. 确保您的细胞健康，并确保在细胞洗涤过程中非常轻柔地处理它们。We also recommend limiting the room temperature incubation time of the cell suspension with Concanavalin A beads to 5 minutes. Resting the tubes at the appropriate incubation temperatures in lieu of rotating or rocking the tubes during the incubations for cell-bead binding, antibody binding, and pAG-Tn5 enzyme binding helps to mitigate bead clumping and does not have any adverse effects on sequencing depth, identified peak numbers, or binding patterns.

尽管如此，我们的内部测试已经证明，珠结块并未不利影响 CUT&Tag 实验的最终结果。

Last updated: 2024 年 9 月 12 日