我是否应该担心我的 Concanavalin A 磁珠在我的一些 CUT&RUN 实验中“聚集在一起”?

Concanavalin A 磁珠可以很容易地凝集在一起，尤其是在细胞裂解时。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell wash. 我们还建议将细胞悬液与 Concanavalin A 磁珠的室温孵育时间限制为 5 分钟。Instead of rotating or rocking the tubes during the incubations for cell-bead binding, antibody binding, and pAG-MNase enzyme binding, you may also let the tubes rest at the appropriate incubation temperatures. This seems to help to mitigate the beads from clumping and does not have any adverse effects on the final results. 尽管如此，我们的内部测试表明，珠子结块不会对 CUT&RUN 实验的最终结果产生负面影响。

Last updated: 2024 年 9 月 12 日