NG-seq 分析需要多少 CUT&RUN DNA?

The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. 每个反应的 CUT&RUN DNA 的典型产量为 0.6 至 6 ng。Our SimpleChIP^® ChIP-seq DNA Library Prep Kit for Illumina^® #56795 will work with starting DNA as low as 0.5 ng. 如果起始量太低，您也可以将 PCR 循环数增加到 20，以增加库的扩增。

Last updated: 2024 年 9 月 12 日