CST CUT&Tag 试剂盒是否对分离的胞核奏效？

我们的 CUT&Tag 试剂盒经优化用于完整细胞，并且适用于庞大数目的细胞系，贴壁细胞和悬浮细胞皆适用。While our kit works with pre-isolated nuclei because the Concanavalin A beads bind to glycoproteins on the surface of both cells and nuclei, we haven't found that pre-isolation of cell nuclei helps to increase the CUT&Tag signal. 仅对于毛地黄皂苷未高效渗透的细胞如酵母细胞或植物细胞（二者均含细胞壁），才应当需要胞核预分离。

Last updated: 2024 年 9 月 12 日