Do you recommend performing size selection of the DNA during library preparation for CUT&RUN or ChIP-seq?

We do not recommend performing size selection of the DNA during library preparation for CUT&RUN or ChIP-seq. 根据我们的经验和客户反馈，大小选择可以显著降低 DNA 文库产率和多样性。If you are concerned about large DNA fragments >1 kb, then simply decrease the extension time when amplifying the adaptor-ligated DNA sample during the library preparation. 我们发现将延伸时间减少到 10 至 15 秒大大减少了大于 1kb 的大片段的扩增，同时足以在 CUT&RUN 库制备过程中扩增较小和更理想的 DNA 片段。

Last updated: 2024 年 9 月 12 日