Can I use Drosophila Spike-In Control Kits for CUT&Tag for my CUT&RUN assay?

Yes. Because the CUT&RUN workflow does not use a species-specific secondary antibody, both the H2Av Rabbit Monoclonal Antibody and the H2Av Mouse Monoclonal Antibody included in the Drosophila Spike-In Control Kits for CUT&Tag are compatible with the CUT&RUN assay for sample normalization purposes.

We recommend selecting the Drosophila Spike-In Control Kit for CUT&Tag based on the species of your test antibody. For example, use the Drosophila Spike-In Control Kit for CUT&Tag (Rabbit) #29811 when your CUT&RUN assay uses a rabbit primary antibody, and use the mouse version #19629 when your primary antibody is mouse. That said, purchasing the Drosophila spike-in materials separately through these CUT&Tag control kits is more expensive than using the CUT&RUN Assay Kit (with Drosophila Spike-In Control) #84647.

Last updated: April 23, 2026