Can I use DNA affinity columns to purify CUT&RUN DNA for transcription factors?

Yes, we recommend using our DNA Purification Buffers and Spin Columns (for ChIP & CUT&RUN) #14209 for purifying CUT&RUN DNA, no matter its histone modifications, transcription factors, or cofactors. In our internal tests, our DNA spin columns effectively recovered DNA fragments greater than, or equal to, 35 bp. Considering >98% CUT&RUN DNA are proven to be longer than 35 bp in length, our DNA spin columns provide a simple, inexpensive, and robust method for purifying your CUT&RUN DNA fragments. 请注意，并非所有的 DNA 旋转柱都能捕获相同范围的 DNA 片段大小，因此，如果您使用其他供应商的 DNA 旋转柱，则需要确定这些柱保留的最小 DNA 片段大小，因为更大的片段大小临界值会对您的结果产生负面影响。

Last updated: 2024 年 9 月 12 日