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高内涵成像资源中心

可靠的 CST® 解决方案，助力您的药物研发之旅

Image of an immunofluorescence assay on untreated SK-MEL-2 cells

Confocal immunofluorescent analysis of untreated SK-MEL-2 cells using SQSTM1/p62 (D5L7G) Mouse Monoclonal Antibody #88588, DyLight® 554 Phalloidin #13054, and DAPI #4083.

在高风险的药物开发领域，能够推动筛选工作稳步推进且可重复的数据是至关重要的。高内涵成像 (HCI) 能在紧迫的时间要求下，规模化获得数据，从而深入揭示复杂的细胞过程，但这只有在使用可靠的抗体时才能实现。CST 提供的适用于高内涵成像的免疫荧光-免疫细胞化学 (IF-IC) 验证抗体均经过严格测试，使您能够：

  • 获得可信数据：消除因非特异性结合导致的假阳性，或因靶标特异性差导致的假阴性。使用高特异性抗体对于图像分割和准确的数据定量至关重要。<br/>
  • 节省宝贵时间：经过 IF-IC 验证的 HCI 级抗体确保适用于您的检测，无需您自行验证。<br/>
  • 避免实验失败：HCI 工作流程本身就已经充满挑战。排除试剂可靠性这一变量，让您能够专注于洞察结果，而不是排查问题。

特异的 HCI 试剂和定制解决方案支持大规模精准图像分析

High-Content Imaging-Ready Antibodies

Select from a broad catalog of validated antibodies guaranteed to deliver reliable results.

Bulk Orders & Lot Reservations

Future-proof your HCI assay with a consistent supply throughout your project.

  • Keep your projects on track and eliminate unplanned interruptions with lot reservations
  • Ensure assay-to-assay consistency over time
  • Bulk orders can be aliquoted and delivered on a preferred schedule to complement your project
  • Ideal for projects with longer time frames

Catalog & Custom Antibody Conjugation Solutions

Leverage CST conjugation expertise. Flexible options seamlessly fit into your assay.

  • Streamlined ordering from a catalog of 3,200+ validated off-the-shelf or SignalFlex™ on-demand conjugates
  • Custom Conjugation services tailored to deliver the exact antibody-label combination needed for your assay
  • 1,600+ BSA- and azide-free conjugation-ready antibodies are in stock, with most other monoclonals available carrier-free upon request

Application Workflow Reagents

Utilize high-quality reagents from CST throughout your HCI-workflow.

高内涵成像常用标志物

以下仅列出了部分精选的 HCI 级抗体。浏览我们的完整产品组合，了解更多 HCI 级抗体。

细胞器与形态学

  • TOM20（线粒体外膜）<br/>
  • PDI（内质网标志物）<br/>
  • GM130（顺面高尔基体标志物）<br/>
  • Lamin A/C（核膜完整性）<br/>
  • alpha-Tubulin（微管标志物）<br/>
  • LAMP1（溶酶体标志物）<br/>

Image of a confocal immunofluorescent analysis of HeLa cells.

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) Rabbit Monoclonal Antibody #9091 (green), DyLight® 554 Phalloidin #13054, and DRAQ5 #4084 (blue).

增殖与细胞周期

Image of a confocal immunofluorescent analysis of HeLa cells.

Confocal immunofluorescent analysis of HeLa cells using Ki-67 (8D5) Mouse Monoclonal Antibody #9449 (green), DyLight® 554 Phalloidin #13054, and DRAQ5 #4084 (blue).

细胞死亡与应激反应

Confocal immunofluorescent analysis of HeLa cells.

Confocal immunofluorescence of HeLa cells following UV irradiation. Double-strand DNA breaks were detected using Phospho-Histone H2A.X (Ser139) (D7T2V) Mouse Monoclonal Antibody #80312 (green), and the actin cytoskeleton was counterstained with DyLight® 554 Phalloidin #13054 (red).

信号转导与转位

Confocal immunofluorescent analysis of HT-1080 cells.

Confocal immunofluorescence analysis of HT-1080 cells stimulated with hTNF-α #8902 (20 ng/ml, 20 min). Cells were probed with NF-κB p65 (D14E12) Rabbit Monoclonal Antibody #8242 (green), demonstrating robust nuclear translocation. Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red), and DNA was stained with DRAQ5 #4084 (blue).

未找到适用于您研究靶标的 HCI 级抗体？ 提交产品开发申请。

高内涵成像常见问题解答

展开所有链接

什么是高内涵成像？

高内涵成像与传统荧光显微镜有何不同？

高内涵成像通常需要哪些染色试剂？

如何判断 CST 一抗是否为 HCI 级？

为什么抗体特异性对高内涵成像至关重要？

细胞涂色是什么？与基于抗体的高内涵筛选 (HCS) 相比，其局限性是什么？

高内涵成像、高内涵分析和高内涵筛选之间有什么区别？

DyLight 是 Thermo Fisher Scientific, Inc. 及其子公司的注册商标。