Goat Anti-Rabbit IgG, Fc Fragment Antibody (Alexa Fluor® 594 Conjugate) #70872
Filter:
- IF
Product Specifications
| REACTIVITY | Rab |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- Rab-Rabbit
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 1.5 mg/mL.
Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:800 – 1:3200 should yield acceptable results for immunofluorescent assays.
When using rabbit Fc-directed secondaries, a higher concentration of primary antibody may be required. The optimal dilution of each primary antibody should be determined by titration.
When using rabbit Fc-directed secondaries, a higher concentration of primary antibody may be required. The optimal dilution of each primary antibody should be determined by titration.
Storage
Supplied lyophilized with a formulation of 0.01 M sodium phosphate, 0.25 M sodium chloride (pH 7.6), 15 mg/mL BSA, and 0.05% azide at 4ºC. Reconstitute lyophilized material with 0.75 mL dH2O and centrifuge until clear. The product is stable for six weeks at 4ºC as an undiluted liquid. For extended storage, aliquot and freeze at -80ºC, or add an equal volume of glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles. Protect from light.
Specificity / Sensitivity
Goat Anti-Rabbit IgG, Fc Fragment Antibody (Alexa Fluor® 594 Conjugate) reacts with the Fc portion of Rabbit IgG heavy chain, but does not cross-react with the Fab portion of rabbit immunoglobulins. This antibody conjugate also has minimal cross-reaction with human serum proteins and may cross-react with immunoglobulins from other species; however, it does not react with non-immunoglobulin serum proteins.
Species Reactivity:
Rabbit
Background
This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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