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Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate)

Anti-mouse IgG (H+L), F(ab')₂ Fragment (PE Conjugate) #8887

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Product Name

59997 Anti-mouse IgG (H+L), F(ab')₂ Fragment (PE Conjugate)

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Supporting Data

REACTIVITY	M
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

Anti-mouse IgG (H+L), F(ab')₂ Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')₂ fragment results in less non-specific binding to cells through Fc receptors.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.

Storage

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

F(ab')₂ fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Species Reactivity:

Mouse

Background

This product has been optimized for use as a secondary antibody in FLOW ctytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

Alternate Names

anti mouse; PE; R-PE; secondary

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8887