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  3. Wnt5a/b (C27E8) Rabbit Monoclonal Antibody
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Wnt5a/b (C27E8) Rabbit Monoclonal Antibody #2530

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    Wnt5a/b (C27E8) Rabbit Monoclonal Antibody detects endogenous levels of Wnt5a and Wnt5b proteins. This antibody cross-reacts with an unidentified protein between 55 and 60 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu201 of Isoform 1 and Glu186 of Isoform 2 of the human Wnt5a protein. This antibody works to detect both isoforms of human Wnt5a protein.

    Background

    The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5).
    Wnt-5a has been shown to signal through the canonical Wnt pathways as well as through non-canonical pathways and is up-regulated in various types of human cancers (6-8). In melanoma, Wnt5a is thought to directly affect cell motility and metastasis (9).

    Alternate Names

    epididymis secretory sperm binding protein; hWNT5A; MGC2648; Protein Wnt-5a; Protein Wnt-5b; wingless-type MMTV integration site family, member 5A; wingless-type MMTV integration site family, member 5B; Wnt family member 5A; Wnt family member 5B; WNT-5A protein; WNT-5B protein; WNT5A; WNT5B

    Database Links

    UniProt ID: Q9H1J7 , P41221


    Entrez-Gene Id: 81029 , 7474


    限制使用

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    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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