Render Target: STATIC
Render Timestamp:
6/4/2026, 9:00:52 PM EDT
6/5/2026, 1:00:52 AM UTC
Commit: 33ae406c5df2bbc7a7d3c17605228fbeff8ea416
XML generation date: 2025-11-12 16:21:06.198
Product last modified at: 2026-03-20T08:04:56.094Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
Trial Size Available Flag

Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody #28307

Filter:
  • WB
  • IP
  • F

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa) 55
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.

    Background

    The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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