Name: TRIM33 (F9P6E) Rabbit Monoclonal Antibody
Price: 178.00 USD
Availability: InStock

Filter:

WB
IP
IHC
F

Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	150
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

TRIM33 (F9P6E) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM33 protein. This antibody is not recommended for use with mouse samples using flow cytometry.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TRIM33 protein.

Background

The transcriptional intermediary factor 1 (TIF1) family represents a group of proteins with multiple histone-binding domains. In humans, this family comprises four proteins, TIF1α/TRIM24, TIF1β/TRIM28/KAP1, TIF1γ/TRIM33/Ectodermin, and TIF1δ/TRIM66, which are characterized by an amino-terminal tripartite motif (TRIM) domain consisting of a RING domain, two B boxes, a coiled-coil domain, and a carboxy-terminal PHD finger and bromodomain (1). Despite their similar overall structure, these proteins have diverse roles in transcriptional regulation. TIF1α functions as a ligand-dependent nuclear receptor coregulator and more recently has been implicated in regulating p53 stability (2). TIF1β is an intrinsic component of the N-CoR1 corepressor complex and the NuRD nucleosome-remodeling complex (3); it functions as a corepressor for Krüppel-associated box (KRAB) zinc-finger transcription factors (4). Furthermore, TIF1β promotes heterochromatin-mediated gene silencing formation by serving as a cofactor for heterochromatin protein HP1 (5). TIF1δ expression is restricted to the testis and has been shown to interact with HP1γ (6).

Alternate Names

8030451N04Rik; AI413936; DKFZp586K1123; E3 ubiquitin-protein ligase TRIM33; Ect; Ecto; ectode; Ectodermin homolog; Kiaa1113; mKIAA1113; RING-type E3 ubiquitin transferase TRIM33; Tif1; TIF1-gamma; Tif1g; Transcription intermediary factor 1-gamma; TRI33; Trim33; tripartite motif protein 33; tripartite motif-containing 33; Tripartite motif-containing protein 33

Database Links

UniProt ID: Q99PP7

Entrez-Gene Id: 94093

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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TRIM33 (F9P6E) Rabbit Monoclonal Antibody #46147