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  3. TRAP/ACP5 (F7J8N) Rabbit Monoclonal Antibody
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TRAP/ACP5 (F7J8N) Rabbit Monoclonal Antibody #34306

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  • WB

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 35, 17
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    TRAP/ACP5 (F7J8N) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRAP/ACP5 protein. This antibody detects both the proenzyme (TRAP 5a) and the mature enzyme (TRAP 5b).

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn131 of human TRAP/ACP5 protein.

    Background

    Tartrate-resistant acid phosphatase type 5 (TRAP/ACP5) is a metalloenzyme synthesized as a roughly 35 kDa proenzyme, TRAP 5a, that is later processed into a 17 kDa mature or active form, TRAP 5b (1-3). TRAP 5b serum levels correlate to osteoclast numbers, though TRAP/ACP5 is also expressed in other cell types, including macrophages and dendritic cells (4,5). TRAP/ACP5 plays an essential role in regulating bone resorption by dephosphorylating osteopontin to promote osteoclast migration (6). In macrophages, TRAP/ACP5 helps regulate the secretion of inflammatory cytokines (7). In dendritic cells, TRAP/ACP5 is important for maturation (8). TRAP/ACP5 can also promote breast cancer metastasis and pulmonary fibrosis through activation of Smad2/3, TGFβR2, and β-catenin signaling (9,10).

    Alternate Names

    acid phosphatase 5, tartrate resistant; ACP5; HPAP; human purple acid phosphatase; MGC117378; PPA5; Tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5a; tartrate-resistant acid phosphatase 5b; Tartrate-resistant acid phosphatase type 5; TR-AP; TRACP5a; TRACP5b; TRAP; TrATPase; Type 5 acid phosphatase

    Database Links

    UniProt ID: P13686


    Entrez-Gene Id: 54


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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