Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TDP43 (D9R3L) Horse Chimeric Monoclonal Antibody #73436
Filter:
- IF
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Horse chimeric IgG4 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as TDP43 (D9R3L) Rabbit Monoclonal Antibody #89789.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
TDP43 (D9R3L) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total TDP43 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from TDP43 (D9R3L) Rabbit Monoclonal Antibody #89789 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, TDP43 (D9R3L) Rabbit Monoclonal Antibody #89789, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly282 of human TDP43 protein.
The parent antibody, TDP43 (D9R3L) Rabbit Monoclonal Antibody #89789, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly282 of human TDP43 protein.
Background
TDP43 (TAR DNA-binding protein 43) is involved in transcriptional regulation and exon splicing (1,2). While normal TDP43 is a nuclear protein, pathological TDP43 is a component of insoluble aggregates in patients with frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). In these disorders, TDP43 is abnormally ubiquitinated, phosphorylated, and cleaved to generate carboxy-terminal fragments that are sequestered as insoluble aggregates in neuronal nuclei, perikarya, and neurites (3,4). Additionally, TDP43 inhibits the expression of the HIV-1 gene and regulates CFTR gene splicing (1,5).
Alternate Names
ALS10; TADBP; TAR DNA binding protein; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TARDBP; TDP-43; TDP43
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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