Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TDP1 (D8D1B) Rabbit Monoclonal Antibody #59710
Filter:
- WB
- IF
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
TDP1 (D8D1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total TDP1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro587 of human TDP1 protein.
Background
Topoisomerases are ubiquitous, conserved enzymes that remove DNA supercoils resulting from processes such as chromosome segregation, DNA replication, transcription, and repair (1). Topoisomerase inhibitors such as camptothecin and etoposide trap the enzyme as a DNA-bound intermediate, and these drugs are used to treat multiple human cancers (1,2). Tyrosyl-DNA-phosphodiesterases TDP1 and TDP2 function in the base excision repair (BER) and nonhomologous end joining (NHEJ) DNA repair pathways, respectively, and function in part in the repair of stalled topoisomerase-DNA complexes (3). Research has shown that inhibitors of tyrosyl-DNA-phosphodiesterases may act synergistically with topoisomerase inhibitors, allowing the potential for a more robust treatment of cancer (4,5). In small cell lung cancer, research suggests that TDP1 and topoisomerase 1 levels can predict sensitivity to topoisomerase 1 inhibitors (6).
- Pommier, Y. (2013) ACS Chem Biol 8, 82-95.
- Saijo, N. (2000) Ann N Y Acad Sci 922, 92-9.
- Pommier, Y. et al. (2014) DNA Repair (Amst) 19, 114-29.
- Nguyen, T.X. et al. (2012) J Med Chem 55, 4457-78.
- Huang, S.N. et al. (2011) Expert Opin Ther Pat 21, 1285-92.
- Meisenberg, C. et al. (2014) J Cancer Sci Ther 6, 258-67.
Alternate Names
FLJ11090; MGC104252; TDP1; TYDP1; Tyr-DNA phosphodiesterase 1; Tyrosyl-DNA phosphodiesterase 1
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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