Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Synapsin-1 (D12G5) Mouse Chimeric Monoclonal Antibody #31621
Filter:
- IF
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse chimeric IgG2a |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Synapsin-1 (D12G5) Rabbit Monoclonal Antibody #5297.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
Synapsin-1 (D12G5) Mouse Chimeric Monoclonal Antibody detects endogenous levels of total synapsin protein. The antigen is 100% conserved between human synapsin-1a and synapsin-1b.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from Synapsin-1 (D12G5) Rabbit Monoclonal Antibody #5297 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, Synapsin-1 (D12G5) Rabbit Monoclonal Antibody #5297, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln483 of human synapsin-1 protein.
The parent antibody, Synapsin-1 (D12G5) Rabbit Monoclonal Antibody #5297, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln483 of human synapsin-1 protein.
Background
Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).
Alternate Names
Brain protein 4.1; MRX50; SYN1; SYN1a; SYN1b; Synapsin; Synapsin I; synapsin Ib; Synapsin-1; SYNI
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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