SMARCD1/BAF60A (E7W9W) Rabbit Monoclonal Antibody #35070

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8).

SMARCD/BAF60 proteins are BAF family members that, along with SMARCC1/BAF155A, serve as an initial subcomplex critical for the formation of the intact BAF complex (9). These proteins serve as an interface between the BAF complex and many other key transcription factors, including glucocorticoid receptor and p53 (10,11). SMARCD1/BAF60A has been implicated in stem cell differentiation by controlling H3K27me3 distribution (12). Furthermore, SMARCD1/BAF60A has been shown to play a role in Notch signaling in glioblastomas (13).