SLBP Antibody

Filter:

WB

Inquiry Info. # 12087

Please see our recommended alternatives.

Product Specifications

REACTIVITY	H M R Dg
SENSITIVITY	Endogenous
MW (kDa)	40
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Dg-Dog

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

SLBP Antibody recognizes endogenous levels of total SLBP protein.

Species Reactivity:

Human, Mouse, Rat, Dog

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg160 of human SLBP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Stem-loop binding protein (SLBP) is the mammalian orthologue of Xenopus SLBP1, a post-transcriptional regulator of histone mRNA. SLBP binds the conserved stem-loop structure at the 3’ end of replication-dependent histone mRNAs. SLBP binding aids in the 3’ end processing of histone pre-mRNA in association with U7 snRNP and the zinc finger protein hZFP100 (1,2), as well as translation and transport of histone mRNA (3).

The expression of replication-dependent histone genes peaks during S phase of the cell cycle. Mammalian SLBP is also cell cycle regulated in most somatic cells, but not in mouse oocytes or embryos, indicating a potential role for SLBP in chromatin organization and remodeling (4).

Phosphorylation of SLBP at Thr61 by CDK1 regulates SLBP stability by targeting the protein for degradation upon completion of DNA synthesis (5). SLBP association with histone mRNA maintains its stability, and is regulated by the peptidyl-prolyl isomerase, Pin1, and PP2A phosphatase (6,7).

Alternate Names

hairpin binding protein, histone; HBP; histone binding protein; Histone RNA hairpin-binding protein; histone stem-loop binding protein; Histone stem-loop-binding protein; SLBP; stem-loop (histone) binding protein; stem-loop binding protein

Database Links

UniProt ID: Q14493

Entrez-Gene Id: 7884

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

SLBP Antibody #12087