SKAR Antibody #3235
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SKAR Antibody detects endogenous levels of total SKAR protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SKAR alpha. Antibodies are purified using protein A and peptide affinity chromotography.
Background
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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