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SHANK3 (D5K6R) Feline Chimeric Monoclonal Antibody

SHANK3 (D5K6R) Feline Chimeric Monoclonal Antibody #67606

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Feline chimeric IgG1

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as SHANK3 (D5K6R) Rabbit Monoclonal Antibody #64555.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

SHANK3 (D5K6R) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total SHANK3 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

This recombinant chimeric antibody is engineered from SHANK3 (D5K6R) Rabbit Monoclonal Antibody #64555 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, SHANK3 (D5K6R) Rabbit Monoclonal Antibody #64555, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1210 of human SHANK3 protein.

Background

The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (1).

Alternate Names

DEL22q13.3; KIAA1650; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; PSAP2; SCZD15; SH3 and multiple ankyrin repeat domains 3; SH3 and multiple ankyrin repeat domains protein 3; SHAN3; shank postsynaptic density protein; SHANK3; shank3 postsynaptic density protein; SPANK-2

Database Links

UniProt ID: Q9BYB0

Entrez-Gene Id: 85358

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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