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  3. SH2D2A/TSAd (E2V4X) Rabbit Monoclonal Antibody
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SH2D2A/TSAd (E2V4X) Rabbit Monoclonal Antibody #50147

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 51, 48
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SH2D2A/TSAd (E2V4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SH2D2A/TSAd protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SH2D2A/TSAd protein.

    Background

    The T cell-specific adapter protein (TSAd), encoded by the SH2D2A gene, is an adapter molecule containing an SRC homology 2 (SH2) domain and a proline-rich region (PRR) in addition to several tyrosine phosphorylation sites (1). The protein is predominantly expressed in T cells, NK cells, and endothelial cells (2,3). TSAd is required for T cell polarity establishment, interaction with antigen-presenting cells (4), and maintenance of T regulatory cell (Treg) function (5). The protein modulates T cell receptor (TCR) signaling in activated T cells via interaction with the tyrosine kinases Lck (6) and Itk (7). In endothelial cells, TSAd is required for VEGF-induced angiogenic sprouting through its binding to phosphorylated VEGF Receptor 2 (VEGFR2) at Tyr951 and coupling of VEGFR2 signaling to c-Src activation (8,9).

    Alternate Names

    F2771; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; SH2 domain-containing protein 2A; SH22A; SH2D2A; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAD; VEGF receptor-associated protein; VRAP

    Database Links

    UniProt ID: Q9NP31


    Entrez-Gene Id: 9047


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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