Name: RUNX2 (D1L7F) Rabbit Monoclonal Antibody
Price: 351.00 USD
Availability: InStock

Filter:

WB
IP
F
ChIP

Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	55-62
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
F-Flow Cytometry
ChIP-Chromatin Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Flow Cytometry (Fixed/Permeabilized)	1:1600 - 1:6400
Chromatin IP	1:100
Chromatin IP-seq	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #68007.

实验步骤

Specificity / Sensitivity

RUNX2 (D1L7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total RUNX2 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala273 of human RUNX2 protein.

Background

Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters (1-3). RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) (4-6). RUNX2 is also abnormally expressed in various human cancers, including prostate and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells (7-10).

Alternate Names

Acute myeloid leukemia 3 protein; AML3; CBF-alpha 1; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120022; MGC120023; Oncogene AML-3; OSF-2; OSF2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEA2aA; PEBP2-alpha A; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; polyomavirus enhancer binding protein 2 alpha A subunit; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt related transcription factor 2; Runt-related transcription factor 2; RUNX family transcription factor 2; RUNX2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

Database Links

UniProt ID: Q13950

Entrez-Gene Id: 860

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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RUNX2 (D1L7F) Rabbit Monoclonal Antibody #12556