PhosphoPlus® Rb (Ser780, Ser795, Ser807/811) Antibody Kit #9300
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Product Information
Kit Usage Information
Protocols
- 7003:
- 7074: Western Blotting
- 7075: Western Blotting
- 7727:
- 9301: Western Blotting, Immunoprecipitation (Magnetic)
- 9303:
- 9307: Western Blotting, Immunoprecipitation (Magnetic)
- 9308: Western Blotting, Immunoprecipitation (Agarose)
- 9309: Western Blotting, Fluorescent Western, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic
Product Description
The PhosphoPlus® Rb (Ser780, Ser795, Ser807/811) Antibody Kit provides reagents and protocols for the rapid analysis of Rb phosphorylation.
Specificity / Sensitivity
Phospho-Rb (Ser780, Ser795, Ser807/811) Antibodies detect endogenous levels of Rb only when phosphorylated at the target sites. The monoclonal Rb antibody does not recognize the Rb homologues p107 or p130, or other proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser780, Ser795 or Ser807/811 of human Rb. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a fusion protein (Rb-C Fusion Protein #6022) containing residues 701-928 of human Rb .
Background
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
- Sherr, C.J. (1996) Science 274, 1672-7.
- Nevins, J.R. (1992) Science 258, 424-9.
- Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
- Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
- Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
- Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
- Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
Alternate Names
p105-Rb; pp110; retinoblastoma
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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