The Plasma Membrane Marker Antibody Sampler Kit provides an economical means of detecting plasma membrane markers in various cell types. 该试剂盒包含的抗体足以使用每种一抗进行两次蛋白印迹实验。
Each antibody in the Plasma Membrane Marker Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb recognizes endogenous levels of Na,K-ATPase α1 protein only when phosphorylated at Tyr10, and cross-reacts with an induced 75-80 kDa doublet of unknown origin. Pan-Cadherin (28E12) Rabbit mAb 可检测内源水平的总钙黏素蛋白。4F2hc/CD98 (D3F9D) XP® Rabbit mAb recognizes endogenous levels of total 4F2hc/CD98 protein, and is predicted to detect multiple isoforms of 4F2hc/CD98.
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro12 of human Na,K-ATPase α1, Pro780 of human E-cadherin, Arg526 of human N-cadherin, Val509 of human 4F2hc/CD98, Glu20 of human caveolin-1, Leu520 of human ENPP1, a conserved region of human N-, R-, E- and P-cadherin proteins, or a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of human Na,K-ATPase α1 protein.
Na,K-ATP 酶属于 P 型 ATP 酶家族的整合型膜异二聚体。Phosphorylation of Na,K-ATPase at Tyr10 has been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters (1). 钙黏着蛋白是在其胞外结构域中含有大约 100 个残基的钙黏着蛋白重复序列的跨膜糖蛋白超家族。Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells (2). 4F2hc 是一种溶质载体家族的跨膜蛋白。4F2hc forms heterodimeric complexes with various amino acid transporters, such as LAT1 and LAT2, and regulates uptake of amino acids (3). 21-24 kDa 整合蛋白 caveolins 是胆固醇/神经鞘脂富含的质膜微区小窝的主要结构组分。Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions (4). 核苷酸内焦磷酸酶/磷酸二酯酶 1 (ENPP1) 是一种单道 II 类跨膜蛋白，主要参与质膜的 ATP 水解作用。ENPP1 plays important roles in bone mineralization and soft tissue calcification (5).