Pim Kinase Antibody Sampler Kit #9779
Product Information
Kit Usage Information
Protocols
- 3247: Western Blotting
- 4165: Western Blotting
- 4730: Western Blotting, Immunoprecipitation (Magnetic)
- 5284: Western Blotting, Immunohistochemistry (Paraffin), Flow
- 7074: Western Blotting
- 9239: Western Blotting
Product Description
The Pim Kinase Antibody Sampler Kit provides an economical means to detect all three Pim kinases along with Bad and Phospho-Bad (Ser112). The kit contains enough primary and secondary antibody to perform two western blot experiments.
Background
Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).
The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).
The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).
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- Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
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- Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
- Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
- Pasqualucci, L. et al. (2001) Nature 412, 341-6.
- Kim, O. et al. (2004) Oncogene 23, 1838-44.
- Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
- Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
- Saris, C.J. et al. (1991) EMBO J 10, 655-64.
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