Phospho-(Ser) CDKs Substrate Antibody #2324
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- WB
- IP
Inquiry Info. # 2324
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Product Specifications
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Peptide ELISA (DELFIA) | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
Phospho-(Ser) CDKs Substrate Antibody detects phospho-serine in a (K/R)(S*)PX(K/R) motif. This antibody is phospho-specific but does not recognize phospho-serine in the absence of the CDK motif. This antibody does not cross-react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins.
Species Reactivity:
All Species Expected
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic phospho-CDK substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.
Background
Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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