Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-Rab10 (Thr73) (F3X5B) Rabbit Monoclonal Antibody #48975
Filter:
- WB
- IP
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
Phospho-Rab10 (Thr73) (F3X5B) Rabbit Monoclonal Antibody recognizes endogenous levels of Rab10 protein only when phosphorylated at Thr73. This antibody does not cross-react with other Rab proteins. This antibody detects a 300 kDa protein of unknown identity in some cell lines.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr73 of human Rab10 protein.
Background
Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc) and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8).
Leucine-rich repeat kinase 2 (LRRK2) phosphorylates Rab10 at Thr73. LRRK2 hyperactivity, driven by pathogenic mutations or by the vacuolar protein sorting-associated protein 35 (VPS35) D620N Parkinson's disease (PD) associated mutation increases Rab10 phosphorylation at Thr73. Conversely, LRRK2 inhibition decreases Rab10 phosphorylation at this site, making it a key readout for LRRK2 activity (9-11).
Leucine-rich repeat kinase 2 (LRRK2) phosphorylates Rab10 at Thr73. LRRK2 hyperactivity, driven by pathogenic mutations or by the vacuolar protein sorting-associated protein 35 (VPS35) D620N Parkinson's disease (PD) associated mutation increases Rab10 phosphorylation at Thr73. Conversely, LRRK2 inhibition decreases Rab10 phosphorylation at this site, making it a key readout for LRRK2 activity (9-11).
- Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
- Burton, J.L. et al. (1994) EMBO J 13, 5547-58.
- Roland, J.T. et al. (2009) J Biol Chem 284, 1213-23.
- Chen, Y. et al. (2009) Biochem J 422, 229-35.
- Schuck, S. et al. (2007) Traffic 8, 47-60.
- Babbey, C.M. et al. (2006) Mol Biol Cell 17, 3156-75.
- Sano, H. et al. (2007) Cell Metab 5, 293-303.
- Larance, M. et al. (2005) J Biol Chem 280, 37803-13.
- Thirstrup, K. et al. (2017) Sci Rep 7, 10300.
- Lis, P. et al. (2018) Biochem J 475, 1-22.
- Karayel, Ö. et al. (2020) Mol Cell Proteomics 19, 1546-1560.
Alternate Names
GTP-binding protein RAB10; RAB10; RAB10, member RAS oncogene family; ras-related GTP-binding protein; Ras-related protein Rab-10
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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