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Phospho-HS1 (Tyr397) Antibody

Phospho-HS1 (Tyr397) Antibody #4507

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Inquiry Info. # 4507

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	80
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

Phospho-HS1 (Tyr397) Antibody detects the endogenous level of HS1 protein when phosphorylated at Tyr397.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human HS1. Antibodies are purified using peptide affinity chromatography

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).
Tyr397 of HS1 protein is rapidly phosphorylated by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).

Alternate Names

cortactin-like; CTTNL; HCLS1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS1; LckBP1; p75

Database Links

UniProt ID: P14317

Entrez-Gene Id: 3059

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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