Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-GSK-3 beta (Tyr216)/GSK-3 alpha (Tyr279) (F4F9F) Rabbit Monoclonal Antibody #71057
Filter:
- WB
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 51, 46 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
Phospho-GSK-3 beta (Tyr216)/GSK-3 alpha (Tyr279) (F4F9F) Rabbit Monoclonal Antibody recognizes endogenous levels of GSK-3β and GSK-3α proteins only when phosphorylated at Tyr216 or Tyr279, respectively. This antibody may cross-react with other tyrosine-phosphorylated RTKs.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Y216 of human GSK-3β protein.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
GSK-3β undergoes autophosphorylation at Tyr216 within its activation loop, increasing and stabilizing its kinase activity (6).
GSK-3β undergoes autophosphorylation at Tyr216 within its activation loop, increasing and stabilizing its kinase activity (6).
Alternate Names
glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta; GSK3beta isoform; Serine/threonine-protein kinase GSK3B
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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