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Phospho-cdc25C (Ser198) Antibody

Phospho-cdc25C (Ser198) Antibody #9529

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Product Specifications

REACTIVITY	H
SENSITIVITY	Transfected Only
MW (kDa)	75
SOURCE	Rabbit

Application Key:

WB-Western Blotting
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Flow Cytometry (Fixed/Permeabilized)	1:25

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

Phospho-cdc25C (Ser198) Antibody detects levels of cdc25C only when phosphorylated at Ser198 and only when derived from a transfected DNA construct.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser198 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).
During prophase, polo-like kinase 1 (PLK1) phosphorylates cdc25C at Ser198, causing translocation from the cytoplasm to the nucleus, where cdc25C can interact with cdc2/cyclin B to allow for progression through the remaining stages of mitosis (6).

Alternate Names

CDC25; CDC25C; cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; M-phase inducer phosphatase 3; mitosis inducer CDC25; MPIP3; phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60

Database Links

UniProt ID: P30307

Entrez-Gene Id: 995

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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