Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-ATP-Citrate Lyase (Ser455) (F4T8R) Rabbit Monoclonal Antibody #97366
Filter:
- WB
- IP
- IHC
- F
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
Phospho-ATP-Citrate Lyase (Ser455) (F4T8R) Rabbit Monoclonal Antibody recognizes endogenous levels of ATP-citrate lyase protein only when phosphorylated at Ser455 (equivalent to Ser454 in rat). Nonspecific plasma membrane staining was observed in a subset of tissues by immunohistochemistry.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser455 of human ATP-citrate lyase protein.
Background
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for gluconeogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).
ATP-citrate lyase is also critical in the pathogenesis of Parkinson’s disease (PD). α-synuclein mutations activate ATP-citrate lyase, leading to elevated cytoplasmic p300 activity. Hyperactivation of cytoplasmic p300 subsequently promotes mTORC1 signaling to inhibit autophagy, causing the aggregation of α-synuclein (6).
ATP-citrate lyase is also critical in the pathogenesis of Parkinson’s disease (PD). α-synuclein mutations activate ATP-citrate lyase, leading to elevated cytoplasmic p300 activity. Hyperactivation of cytoplasmic p300 subsequently promotes mTORC1 signaling to inhibit autophagy, causing the aggregation of α-synuclein (6).
- Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33.
- Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79.
- Hughes, K. et al. (1992) Biochem J 288 ( Pt 1), 309-14.
- Pierce, M.W. et al. (1982) J Biol Chem 257, 10681-6.
- Berwick, D.C. et al. (2002) J Biol Chem 277, 33895-900.
- Son, S.M. et al. (2025) Neuron, 1908-1924.e13. doi: 10.1016/j.neuron.2025.03.028.
Alternate Names
ACL; ACLY; ATP citrate lyase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.