PHF5A (F1P9L) Rabbit Monoclonal Antibody #89567

PHF5A, also known as SAP14B, is a highly conserved nuclear PHD domain-containing protein that is a critical component of the U2 small nuclear ribonucleoprotein (U2 snRNP) and plays an important role in the splicing of pre-mRNA that involves the removal of introns and the joining of exons to form mature mRNA (1-3). The assembly and proper recognition of splice sites are driven by sequences at the pre-mRNA intron-exon splice sites. The 5’ splice donor site is recognized by the U1 snRNP complex, while U2 snRNP binds to the 3’ splice site (branch point), ensuring the anchoring of the spliceosome machinery at the splice sites (4,5). Mutations in PHF5A are lethal, as the protein serves an essential role in stabilizing the SF3b spliceosomal complex and linking it to histones to promote alternative splicing (6). PHF5A is overexpressed in many types of cancers, where it promotes growth factors and suppresses apoptotic signals (6-8). PHF5A has also been shown to be a critical regulator in pluripotency as well as neuronal development (9,10).