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  3. PATZ1 (F2N4R) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PATZ1 (F2N4R) Rabbit Monoclonal Antibody #24202

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  • WB
  • IP
  • ChIP

    Product Specifications

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    PATZ1 (F2N4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PATZ1 protein. This antibody may detect a 50 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PATZ1 protein.

    Background

    POZ/BTB and AT hook containing zinc finger 1 (PATZ1) is a transcription factor that regulates gene expression activation or repression depending on the cellular context (1-3). The human PATZ1 gene has four alternatively spliced variants that give rise to structurally similar isoforms. PATZ1 protein contains a BTB domain that facilitates protein interaction and AT hook and zinc finger domains that enable its DNA binding (4,5). PATZ1 has been shown to play key regulatory roles in embryonic development and stem cell identity, proliferation, senescence, and apoptosis (6-10). It has also been reported that PATZ1 plays a critical role in cancer cells, where it appears to carry out both oncogenic and anti-tumor activities (8-10). Notably, PATZ1 is downregulated in thyroid cancer and, thus, thought to function as a tumor suppressor in these cells (11).

    Alternate Names

    BTB-POZ domain zinc finger transcription factor; BTB/POZ domain zinc finger transcription factor; dJ400N23; MAZ-related factor; MAZR; PATZ; PATZ1; POZ (BTB) and AT hook containing zinc finger 1; POZ-, AT hook-, and zinc finger-containing protein 1; POZ-AT hook-zinc finger protein; POZ/BTB and AT hook containing zinc finger 1; Protein kinase A RI subunit alpha-associated protein; RIAZ; ZBTB19; Zinc finger and BTB domain-containing protein 19; Zinc finger protein 278; Zinc finger sarcoma gene protein; ZNF278; ZSG

    Database Links

    UniProt ID: Q9HBE1


    Entrez-Gene Id: 23598


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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