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Pan-Actin (D18C11) Rabbit Monoclonal Antibody

Pan-Actin (D18C11) Rabbit Monoclonal Antibody #8456

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Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	45
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:200
Immunofluorescence (Immunocytochemistry)	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #99736.

Protocol

Specificity / Sensitivity

Pan-Actin (D18C11) Rabbit Monoclonal Antibody recognizes endogenous levels of total actin protein (all isoforms).

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7).

Alternate Names

AAT6; ACT; ACTA; ACTA1; ACTA2; ACTA3; ACTB; ACTC; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTH; actin alpha 1, skeletal muscle; actin alpha 2, smooth muscle; actin alpha cardiac muscle 1; actin beta; actin gamma 1; actin gamma 2, smooth muscle; actin-like protein; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac muscle 1; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; actin, gamma 1; actin, gamma 2, smooth muscle, enteric; Actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; ACTL3; ACTS; ACTSA; ACTSG; ACTVS; alpha 2 actin; alpha skeletal muscle actin; alpha smooth muscle Actin; alpha-actin 3; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; alpha-cardiac actin; Alpha-cardiac actin; ASD5; ASMA; beta actin; beta cytoskeletal actin; Beta-actin; BRWS1; Cell growth-inhibiting gene 46 protein; CFTD; CFTD1; CFTDM; CMD1R; CMH11; cytoskeletal gamma-actin; DFNA20; DFNA26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; growth-inhibiting gene 46; HEL-176; I(2)-actin; LVNC4; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; PS1TP5-binding protein 1; PS1TP5BP1; SHPM; smooth muscle actin; smooth muscle gamma actin; Smooth muscle gamma-actin; VSCM

Database Links

UniProt ID: P60709 , P68133 , P63261 , P68032 , P62736 , P63267

Entrez-Gene Id: 60 , 58 , 71 , 70 , 59 , 72

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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