Name: NOTUM (F1E9C) Rabbit Monoclonal Antibody
Price: 376.00 USD
Availability: InStock

Filter:

WB

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	55,62
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

NOTUM (F1E9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total NOTUM protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His422 of human NOTUM protein.

Background

NOTUM is an extracellular carboxylesterase and a key negative regulator of the Wnt signaling pathway. The palmitoleoylation of Wnt is required for its binding to Frizzled receptors, which is essential for Wnt signaling activation. NOTUM removes the palmitoleate moiety on Wnt ligands to inactivate Wnt signaling (1,2). The function of NOTUM is dependent on glypicans, which bind both NOTUM and Wnt to co-localize them at the cell surface. NOTUM is expressed in the liver (3), bone (4), epithelial cells in the intestine (5), and brain (6) to regulate tissue formation and function. Biological aging may result from increased NOTUM expression, while inhibition of NOTUM may rejuvenate stem cells to promote tissue regeneration (5). In colorectal cancer and gastric cancer, aberrant high NOTUM expression promotes proliferation and metastasis. Knockdown or inhibition of NOTUM activity suppresses its cancer-promoting effects (7-9). Inhibition of NOTUM activity may represent a new approach to treat disease where aberrant NOTUM activity has been identified as the underlying cause (10).

Alternate Names

[Wnt protein] O-palmitoleoyl-L-serine hydrolase; hNOTUM; NOTUM; notum pectinacetylesterase homolog; notum pectinacetylesterase homolog (Drosophila); notum, palmitoleoyl-protein carboxylesterase; Palmitoleoyl-protein carboxylesterase NOTUM; Protein notum homolog

Database Links

UniProt ID: Q6P988

Entrez-Gene Id: 147111

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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NOTUM (F1E9C) Rabbit Monoclonal Antibody #92654