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Mouse IL-3 Neutralizing (D6C1) Rabbit Monoclonal Antibody

Mouse IL-3 Neutralizing (D6C1) Rabbit Monoclonal Antibody #12222

Inquiry Info. # 12222

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Product Specifications

REACTIVITY	M
SENSITIVITY
MW (kDa)
Source/Isotype	Rabbit IgG

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse IL-3 Neutralizing (D6C1) Rabbit mAb has been shown to neutralize the proliferation of IL-3-dependent BaF3 cells in vitro with an ND₅₀ in the range of 0.5-3.5 µg/ml.

Endotoxin

<0.1 EU/µg of antibody

Product Usage Information

CST recommends incubation of the neutralizing antibody with the intended target for 1 hr at 37ºC before addition to the experiment at an optimal concentration determined by the user.

Reconstitution:

Add sterile 10 mM HEPES pH 7.0 to a final concentration of greater than 50 μg/ml. Solubilize for 20 min at room temperature with occasional gentle vortexing.

Application	Dilution
Neutralizing	1:1

Formulation

Lyophilized from a 0.2 µm filtered solution in 10 mM HEPES with trehalose.

Storage

Store lyophilized material at -20ºC. After reconstitution, recommended storage at 4ºC for 1 month or -20ºC for 6 months. Avoid repeated freeze/thawing.

Specificity / Sensitivity

Mouse IL-3 Neutralizing (D6C1) Rabbit Monoclonal Antibody binds to mouse IL-3 and neutralizes its effects in a BaF3 cell proliferation assay. This antibody does not cross-react with human IL-3, human IL-5, human GM-CSF, or mouse GM-CSF.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant mouse IL-3 protein.

Background

IL-3 is produced by T cells, mast cells, and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid, and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support the culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3-specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2 and the PI3K/Akt pathway, and phosphorylation of Stat1/3/5/6 (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3-5).

Alternate Names

colony-stimulating factor, multiple; Hematopoietic growth factor; IL-3; IL3; interleukin 3; interleukin 3 (colony-stimulating factor, multiple); Interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

Database Links

UniProt ID: P08700

Entrez-Gene Id: 3562

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not for Use in Diagnostic Procedures.

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